Catalog Number - 68122-05P

The 68122‑05P Cogent RP PFP™ UHPLC Column is designed for enhanced selectivity of halogenated, aromatic, and polar compounds, providing improved resolution where conventional C18 phases may not fully differentiate analytes.

This stationary phase uses a pentafluorophenyl (PFP) ligand bonded via a four‑carbon linker to high‑purity silica with full double end‑capping, delivering multiple interaction mechanisms including π‑π interactions, dipole interactions, and hydrogen bonding. These combined interactions allow for greater selectivity control and improved separation of structurally similar compounds.

This column is well suited for USP L43 methods and as an alternative phase for new method development requiring different retention behavior.

50 mm Length – Fast Analytical Screening & Throughput

The 50 mm length is optimized for rapid separations and method screening, making it ideal for high‑throughput analytical workflows.

• Short run times and faster gradients

• Efficient selectivity evaluation

• Reduced solvent consumption

✅ Use when reducing run time while maintaining resolution is a priority

Flow Rate & System Requirements (4.6 mm ID, 1.8 µm)

⚠️ Requires UHPLC system capability due to high backpressure

• Typical flow rate range: ~1.0–2.0 mL/min

• Optimal starting range: ~1.2–1.5 mL/min

• Requires high‑pressure system stability

• Standard analytical flow detector compatible

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose PFP for Selective Separations

Enhanced Retention for Halogenated Compounds

• Strong interaction with fluorinated and halogenated analytes

• Improved selectivity vs C18

Multiple Interaction Mechanisms

• π‑π interactions for aromatic compounds

• Dipole and hydrogen bonding interactions

• Greater flexibility during method development

Improved Resolution Without Longer Run Times

• Enables faster methods with maintained or improved separation

• Useful for optimizing existing workflows

Cogent RP PFP™ Advantages

Fully End‑Capped Surface

• Minimizes secondary silanol interactions

• Improves peak symmetry for polar compounds

High Efficiency (1.8 µm Particles)

• High plate counts and peak capacity

• Faster separations vs 3 µm and larger particles

High Purity Silica

• Consistent column performance

• Reliable reproducibility across runs

Typical Applications

• Halogenated compound analysis

• Aromatic compound separations

• Pharmaceutical QA/QC and development

• LC‑MS analytical workflows

• USP L43 method applications

Important Usage Notes

• ⚠️ Requires UHPLC system (high pressure capable)

• Designed for high‑efficiency analytical workflows

• Higher backpressure than larger particle columns

• Use 0.2 µm filtration to maintain column performance